The Baltimore & Ohio Southwestern Railroad (B&O SW) Spring Grove Industrial Track was located in Cincinnati, Ohio and is currently out-of-service.

The Big Four Railroad Depot was located in downtown Springfield, Ohio at Washington and Spring Street. The depot was constructed on the site of one of two Indian mounds in the city. It was demolished in 1969.

The Big Woods, Red River & Lombard Railroad was a narrow gauge railroad in Powell and Menifee counties in Kentucky constructed circa 1910 to access the virgin forests of the Red River valley.

The Brimstone & New River Railroad is a disused railroad from the Cincinnati, New Orleans and Texas Pacific Railway at New River, Tennessee southeast to Lone Mountain. Chartered as the Brimstone Railroad by the W.M. Ritter Lumber company of Virginia, the logging line followed Brimstone Creek and featured lightweight, unballasted track. The line came to fruition after Ritter purchased the New River Lumber Company. The railroad featured a branch within nearly every hollow, with tracks that ascended the mountains via switchbacks. The Brimstone, from Slick Rock north, was paralleled by the earlier Knoxville and New River Railroad (K&NR). The line operated three shay locomotives.

Construction of the Central Ohio Railway, which was proposed to connect Columbus, Ohio to Bellaire, began in June 1850. On November 21, 1866, the Central Ohio concluded a deal with the B&O for a term of 20 years, during which the B&O would operate the railroad.

The former Chesapeake & Ohio Dawkins Subdivision extends from Dawkins, near West Van Lear, to Evanston, Kentucky and was 36 miles long.

The Chesapeake & Ohio Railroad abandoned the steep rail grade that traveled from Ansted to Hawks Nest, West Virginia from 1890 to 1972. Today, the graveled and dirt trail features two trestles and views of the old mines and support functions for the railroad.

Chesapeake & Ohio Railroad Middle Creek Subdivision is owned by CSX Transportation and is located from the Big Sandy Subdivision at Prestonsburg to David, Kentucky. It has not been abandoned, although the line has not been used in years.

The Portsmouth Gaseous Diffusion Plant (USEC), located in Piketon, Ohio, was part of a United States government program to produce highly enriched uranium to fuel nuclear reactors and nuclear weapons. Two railroad spurs were constructed from both the Chesapeake and Ohio and from Norfolk and Western.

The Chesapeake Western Railway is a railroad that once extended from Stokesville, Virginia in Augusta County eastward to Elkton via Harrisonburg. Despite its early potential as a through route between Cincinnati and Washington D.C., and later as a feeder to the coal mines in West Virginia, the railroad never lived up to its potential.

The Chicago, Cincinnati & Louisville Railroad was the shortest route between Cincinnati and Chicago. Today, much of the line has been abandoned.

The Cincinnati and Eastern Railway was a railroad from Idlewild in Norwood, near Cincinnati, to Portsmouth, Ohio. At its western terminus, it connected with the Cincinnati, Lebanon and Northern (CL&N) and the Pennsylvania Railroad Richmond Division (PRR), and to a Norfolk & Western line at its eastern terminus.

The Cincinnati & Muskingum Valley connected the cities of Morrow, Wilmington, Washington Court House, Circleville, Zanesville and Trinway in Ohio.

The Cincinnati & Westwood was a short lived suburban railway from the Cincinnati, Hamilton & Dayton to Westwood, then a suburb of Cincinnati, Ohio. It carried very little traffic from its inception to its closure in 1941.

The Cincinnati, Columbus & Hocking Valley Railroad is a defunct railroad that was later purchased by the Ohio Southern, and was a route extending from Sedalia to Kingman via Jeffersonville, Ohio. It was once proposed as a connection between Columbus and Cincinnati generally along what is now the Interstate 71 corridor.

The Cincinnati & Portsmouth Railway Company was incorporated in March 1873, tasked with constructing a railroad from Cincinnati, Ohio eastward to Portsmouth along the Ohio River. It was later renamed to the Cincinnati, Georgetown and Portsmouth Railroad, although the railroad only reached Russellville, east of Georgetown, before running out of funding.

The Cincinnati, Lebanon & Northern Railway was a railroad in southwest Ohio, connecting Cincinnati to Dayton via Lebanon.

The Cincinnati-owned and financed Cincinnati Southern began operations at King's Mountain, Kentucky, and extended north to Cincinnati and south to Chattanooga, Tennessee. It is part of the Norfolk Southern line today. A modernization project that began in the 1960s has left many tunnels and alignments abandoned.

The Cincinnati Street Connecting Railway was a short 2.5 mile line along Cincinnati, Ohio's riverfront, connecting the Indianapolis & Cincinnati and Little Miami.

The Cedar Avenue substation was constructed in 1917, and was the first automatic substation completed for the Cleveland Railway Company.

The Coal & Coke Railway was a railroad that stretched from Charleston, West Virginia to Elkins, and included branches that connected to many coal fields and coke ovens.

The College Hill Railroad was a line connecting the Cincinnati, Hamilton & Dayton at Spring Grove Avenue in Cincinnati, Ohio, to the College Hill and Mt. Healthy neighborhoods to the north. An extension to Ross was once planned but never constructed. Due to competition from an adjoining streetcar line, the railroad was electrified and became an interurban, before succumbing to financial troubles in 1938.

The Covington and Lexington Railroad later became part of the Louisville and Nashville and then CSX. While it is an active line, the gallery includes the abandoned Paris, Kentucky depot and other scenes from the railroad's past.

The Duluth, South Shore & Atlantic Railway served the upper peninsula of Michigan, namely to carry iron or cooper ore to smelters and docks along the shores of Lake Superior and Lake Michigan.

The Detroit, Toledo & Ironton Railroad is a defunct railroad that had its beginnings as the Iron Railroad Company, which connected Ironton to the coal and timber reserves in southern Ohio. Through acquisitions and mergers, the DT&I stretched for over 370 miles from Ironton to the automobile manufacturing plants in Michigan.

Over the period of a century, the collection of industrial structures from Cedar Avenue south to Thackeray Avenue along Ashland Road in Cleveland, Ohio have been home to a variety of uses and a variety of businesses. Beginning as a power house for the East Cleveland Railroad in 1888, the plant was reused for the Cleveland Ice Machine Company, Westinghouse Electric and Manufacturing and then Thompson Products. Under Thompson, it became part of the Thompson Aircraft Products Company Pump Division, which manufactured pumps and valves for the military and industries in the United States. After Thompson vacated in the mid-1960s, the structures became home to the Virden Manufacturing Company until its abrupt closure in 1979.

The Flemingsburg & Northern Railroad was a standard-gauge railroad that existed from the Louisville & Nashville line at Flemingsburg Junction, Kentucky to Flemingsburg. It was financially plagued and reorganized on seven different occasions before being abandoned in 1955.

An overview of the Hocking Valley Railway, including the Gallipolis, McArthur & Columbus Railroad and the Hocking Valley Scenic Railway in southeast Ohio.

The Kentucky Union Railway was a railroad that extended for 95 miles from Lexington to Jackson, Kentucky. The company was incorporated in 1872 to reach coal and timber resources in the southeastern reaches of the state.

The Lake Superior & Ishpeming Railroad is an iron ore hauler in northern Michigan. All of the lines east of Marquette were abandoned in 1979, with the exception of 5 miles of track that connects with the ex-Duluth, South Shore and Atlantic Railroad to a paper mill in Munising.

Chartered as Ohio's second railroad, the Little Miami connected Cincinnati, Ohio to Xenia and Springfield. It later connected with Columbus. The Little Miami was one of the most profitable railroads in the United States, although its usage and importance declined after World War II. After consolidations and mergers, the Little Miami was dismantled in 1976, and was revived less than a decade later as the longest rail to trail in the United States.

The Louisville, Cincinnati and Lexington Railroad connects Louisville, Kentucky to Cincinnati, Ohio. It later became part of the Louisville & Nashville and is part of the CSX Transportation system today.

The Marietta & Cincinnati Railroad is a defunct railroad that connected Cincnnnati, Ohio to Parkersburg, West Virginia. Through acquisitions during the 1800s, the M&C commanded over 270 miles of railroad, from Cincinnati to Marietta and south to Portsmouth and Hillsboro. The line was later absorbed into the Baltimore & Ohio Southwestern, and some of the route has been dismantled.

Michigan Central Station, located in the Corktown district of Detroit, Michigan, was constructed in mid-1913 for the Michigan Central Railroad at a cost of $15 million. Replacing an earlier passenger rail depot that had burned, the station boasted decent ridership until post-World War II. Attempts were made to sell the station during the 1950s and 1960s due to sharply falling passenger counts, and was nearly closed until Amtrak renovated the station in the 1970s. The last train rolled out in 1988.

The New River, Holston & Western Railroad is a former railroad that extended from Narrows on the New River in Giles County to the village of Suiter in Bland County, Virginia. The 43-mile line followed Wolf Creek or its tributaries for its entire length.

The Norfolk & Western, as part of the Ohio Extension from Elkhorn, West Virginia to Coal Grove, Ohio, snaked through the mountainous canyons throughout the rich coalfields. A part of this alignment, from Lenore to Wayne, West Virginia, was abandoned in 1933 when improvements were made to the line along the Big Sandy River.

The Ohio & Kentucky Railway (O&K) was incorporated by the Kentucky Block Cannel Coal Company, who had leased 5,400 acres of cannel coal in Caney, Morgan County, Kentucky. The near 40 mile railroad extended from just north of Jackson to the Licking River and was active from 1911 until 1933.

The Oneida & Western Railroad is an abandoned rail line between Jamestown and Oneida, Tennessee. The line served vast pockets of virgin timber and coal mines before it was abandoned in 1954 after many years of financial troubles.

The Richmond, Nicholasville, Irvine & Beattyville Railroad (RNI&B, Riney-B) was a railroad that existed between Versailles to Irvine, Kentucky. It was acquired by another railroad in 1899 and extended to Beattyville and Airedale. Another acquisition extended the line west to Frankfort, giving a total mileage of around 110 miles.

Hocking Valley Railway, Wellston & Jackson Belt Railway

The West Virginia Central & Pittsburg Railway was a railroad that once extended from Elkins into the highlands of West Virginia and eventually to Cumberland, Maryland to serve the coal and timber interests.